Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
indoleacetate | 30% inhibition at 10 mM | Pseudomonas sp. | |
mercury compounds | - |
Pseudomonas sp. | |
nicotinate | 65% inhibition at 20 mM | Pseudomonas sp. | |
p-chloromercuribenzoate | - |
Pseudomonas sp. | |
phenylacetate | 79% inhibition at 20 mM | Pseudomonas sp. | |
silver compounds | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | 0.01 | NADH | - |
Pseudomonas sp. | |
0.01 | - |
NADH | spectrophotometric method | Pseudomonas sp. | |
0.01 | - |
Sodium diethyldithiocarbamate | polarographic method | Pseudomonas sp. | |
0.015 | - |
NADH | substrate: sodium diethyldithiocarbamate, polarographic method | Pseudomonas sp. | |
0.02 | - |
O2 | substrate: imidazoleacetate or sodium diethyldithiocarbamate, polarographic method | Pseudomonas sp. | |
0.03 | - |
NADH | polarographic method | Pseudomonas sp. | |
0.3 | - |
imidazoleacetate | spectrophotometric method | Pseudomonas sp. | |
0.5 | - |
imidazoleacetate | polarographic method | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | 90000 | sedimentation equilibrium, gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-imidazoleacetate + NADH + O2 | Pseudomonas sp. | - |
5-hydroxy-4-imidazoleacetate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using protamine sulfate treatment, ammonium sulfate fractionation, chromatography on TEAE-cellulose column, calcium phosphate gel treatment and chromatography on DEAE-Sephadex column | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, potassium phosphate buffer, pH 7.2, no loss of activity for at least 1 month | Pseudomonas sp. |
4°C, 5-100 mM potassium phosphate buffer, pH 7.2, almost full activity retained for several days | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-imidazoleacetate + NAD(P)H + O2 + H+ | electrons can be trasferred from NADH to 2,6-dichloroindophenol in the presence of the enzyme | Pseudomonas sp. | 5-hydroxy-4-imidazoleacetate + NAD(P)+ + H2O | - |
? | |
4-imidazoleacetate + NADH + O2 | - |
Pseudomonas sp. | 5-hydroxy-4-imidazoleacetate + NAD+ + H2O | - |
? | |
imidazolelactate + NADH + O2 + H+ | - |
Pseudomonas sp. | 5-hydroxyimidazolelactate + NAD+ + H2O | - |
? | |
imidazolepropionate + NAD(P)H + O2 + H+ | - |
Pseudomonas sp. | 5-hydroxyimidazolepropionate + NAD(P)+ + H2O | - |
? | |
sodium diethyldithiocarbamate + NADH + O2 | - |
Pseudomonas sp. | ? + NAD+ + H2O | reaction product not identified | ? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
2% loss of activity, 0.1 M potassium phosphate, pH 7.2, 5 min | Pseudomonas sp. |
30 | - |
50% loss of activity, 0.1 M potassium phosphate, pH 7.2, 5 min | Pseudomonas sp. |
40 | - |
95% loss of activity, 0.1 M potassium phosphate, pH 7.2, 5 min | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37.7 | - |
imidazoleacetate | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol per mol enzyme | Pseudomonas sp. | |
FAD | cannot be replaced by FMN or riboflavin | Pseudomonas sp. | |
NADH | - |
Pseudomonas sp. | |
NADPH | beta-NADPH is approximately 13% as active as beta-NADH | Pseudomonas sp. | |
NADPH | alpha-NADH cannot replace beta-NADH at 0.2 mM | Pseudomonas sp. |